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/><span>廠商性質(zhì)：</span>生產(chǎn)廠家</li> <li><img src="/Skins/270236/images/dot.png" /><span>更新時(shí)間：</span>2024-05-13</li> <li><img src="/Skins/270236/images/dot.png" /><span>訪問(wèn) 量：</span>4240</li> </ul> <div id="x4vfcpe" class="pro_detail_btn"> <a href="#order" class="prodtl_btn1">產(chǎn)品咨詢(xún)</a><a href="/contact.html" target="_blank" class="prodtl_btn2">聯(lián)系我們</a> </div> </div> <div id="x4vfcpe" class="clear"></div> </div>  <div class="x4vfcpe" id="ny_pro_box02"> <div class="x4vfcpe" id="con"> <ul id="tags"> <li id="x4vfcpe" class=selectTag><A onmouseover="selectTag('tagContent0',this)" href="javascript:void(0)"onFocus="this.blur()">產(chǎn)品介紹：</A> </li> </ul> <div class="x4vfcpe" id=tagContent> <div id="x4vfcpe" class="tagContent selectTag" id=tagContent0><p>DNA CpG甲基化檢測(cè)</p><p>甲基化特異性的PCR（methylation-specific PCR）應(yīng)用</p><p> DNA甲基化在人的正常發(fā)育、X染色體失活、衰老以及許多人類(lèi)疾?。ㄈ绨l(fā)育畸形、癌癥、心血管疾病、糖尿病和神經(jīng)心理失調(diào)等）過(guò)程中發(fā)揮重要作用，已經(jīng)成為表觀遺傳學(xué)和表觀基因組學(xué)的重要研究?jī)?nèi)容。</p><p> DNA甲基化是表觀遺傳修飾的主要方式，它不改變DNA的一級(jí)結(jié)構(gòu)，卻在細(xì)胞的發(fā)育、基因的表達(dá)、及基因組的穩(wěn)定性中起著重要的作用。CpG島的高甲基化是腫瘤中存在的普遍現(xiàn)象，而啟動(dòng)子CpG島的高甲基化是除突變和缺失外腫瘤中抑癌基因失活的第三種機(jī)制。</p><p> 因此，基因啟動(dòng)子甲基化檢測(cè)在臨床診斷（如甲基化檢測(cè)與低劑量CT相結(jié)合）、藥物敏感性檢測(cè)等方面具有很高的應(yīng)用價(jià)值。</p><p>甲基化特異性PCR法原理（Methylation-specific PCR,MSP） </p><p> MSP法的原理是基于DNA甲基轉(zhuǎn)移酶（DNA methyl-transferase, Dnmt）催化S-腺苷甲硫氨酸作為甲基供體，將胞嘧啶轉(zhuǎn)變?yōu)?-甲基胞嘧啶（mC）的一種反應(yīng)，在真核生物DNA中，5-甲基胞嘧啶是*存在的化學(xué)性修飾堿基。CG二核苷酸是zui主要的甲基化位點(diǎn)，它在基因組中呈不均勻分布，存在高甲基化、低甲基化和非甲基化的區(qū)域，在哺乳動(dòng)物中mC約占C總量的2－7％。一般說(shuō)來(lái)，DNA的甲基化會(huì)抑制基因的表達(dá)；針對(duì)特定區(qū)域設(shè)計(jì)引物，再進(jìn)行PCR擴(kuò)增，甲基化的位點(diǎn)可以被當(dāng)作一個(gè)C/T的SNP來(lái)處理；可利用直接測(cè)序法來(lái)判斷是否CpG位點(diǎn)發(fā)生甲基化。具有方便快捷，靈敏度高的優(yōu)點(diǎn)，是目前較為常用的甲基化檢測(cè)方法。</p><p> MSP實(shí)驗(yàn)流程：</p><p>1.基因組DNA制備，樣本類(lèi)型有：組織，血液，細(xì)胞，毛發(fā)，唾液等。</p><p>2.經(jīng)亞硫酸氫鈉處理后，未甲基化的胞嘧啶發(fā)生脫氨基變?yōu)槟蜞奏?，而甲基化的胞嘧啶則不變。</p><p>3.DNA修飾后，進(jìn)行純化及回收。 </p><p>4.修飾后的DNA進(jìn)行PCR擴(kuò)增。</p><p>5.電泳或直接測(cè)序檢測(cè)</p><p>6.實(shí)驗(yàn)報(bào)告（包括MSP基因組DNA，PCR產(chǎn)物及電泳照片）</p><p>樣品要求<br />1、基因組DNA（≥1µg），純度（OD：1.9-2.0）<br />2、若提供的材料為新鮮組織、血液、細(xì)胞等生物材料，請(qǐng)?zhí)峁┳銐蛱崛?µg以上基因組DNA的材料量。<br />3.樣本運(yùn)輸途中，需要加冰袋，或干冰。</p><p> </p><p> </p></div> <div class="x4vfcpe" id="nr_textbox"><link rel="stylesheet" type="text/css" href="/css/MessageBoard_style.css"> <script language="javascript" src="/skins/Scripts/order.js?v=20210318" type="text/javascript"></script> <a name="order" id="order"></a> <div id="x4vfcpe" class="ly_msg" id="ly_msg"> <form method="post" name="form2" id="form2"> <h3>留言框 </h3> <ul> <li> <h4 class="xh">產(chǎn)品：</h4> <div id="x4vfcpe" class="msg_ipt1"><input class="textborder" size="30" name="Product" id="Product" value="DNA CpG甲基化檢測(cè)服務(wù)" 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