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/><span>更新時(shí)間：</span>2024-05-15</li> <li><img src="/Skins/270236/images/dot.png" /><span>訪問量：</span>10159</li> </ul> <div id="priymv99ks" class="pro_detail_btn"> <a href="#order" class="prodtl_btn1">產(chǎn)品咨詢</a><a href="/contact.html" target="_blank" class="prodtl_btn2">聯(lián)系我們</a> </div> </div> <div id="priymv99ks" class="clear"></div> </div>  <div class="priymv99ks" id="ny_pro_box02"> <div class="priymv99ks" id="con"> <ul id="tags"> <li id="priymv99ks" class=selectTag><A onmouseover="selectTag('tagContent0',this)" href="javascript:void(0)"onFocus="this.blur()">產(chǎn)品介紹：</A> </li> </ul> <div class="priymv99ks" id=tagContent> <div id="priymv99ks" class="tagContent selectTag" id=tagContent0><p>微衛(wèi)星（STR）檢測(cè)</p><p><strong>STR檢測(cè)服務(wù)</strong>STR原理：</p><p>短串聯(lián)重復(fù)序列（short tandem repeat, STR），也稱微衛(wèi)星 DNA（microsalite DNA），是由2 - 6個(gè)堿基對(duì)作為核心單位，串聯(lián)重復(fù)形成的一類DNA序列，由于核心單位重復(fù)數(shù)目在個(gè)體間呈高度變異性并且數(shù)量豐富，構(gòu)成了STR基因座的遺傳多態(tài)性。它分布于人類整個(gè)基因組，平均每15 kb就存在一個(gè)STR基因座。人類基因組內(nèi)已發(fā)現(xiàn)了七千個(gè)以上的STR位點(diǎn)。它具有分布廣泛，易于檢測(cè)，信息量大，有高度多態(tài)性并遵循孟德爾共顯性遺傳等優(yōu)點(diǎn)。是繼限制性片段長(zhǎng)度多態(tài)性（RFLP）之后的第二代遺傳記，目前STR分析已廣泛應(yīng)用于遺傳制圖、連鎖性分析、法醫(yī)學(xué)鑒定、疾病基因定位、物種多態(tài)性和遺傳病的診斷等諸多領(lǐng)域。</p><p><strong style="text-wrap: wrap;">STR檢測(cè)服務(wù)</strong>流程：</p><p>1. 客戶提供</p><p>A.如果提供基因組DNA：至少提供100 ng DNA樣品（針對(duì)每個(gè)多態(tài)性位點(diǎn)，需樣品3-5 ul，濃度為30 - 50 ng/ul）。</p><p>B.如果提供PCR產(chǎn)物：產(chǎn)物要通過預(yù)實(shí)驗(yàn)的電泳檢測(cè)，特異性要好。</p><p>C.如果需要設(shè)計(jì)引物，請(qǐng)?zhí)峁┠康钠涡蛄谢蛘呶⑿l(wèi)星檢測(cè)位點(diǎn)，引物設(shè)計(jì)好后需要客戶確認(rèn)。</p><p>D.除提供樣品類型，DNA濃度和純度值外，還需要注明可能殘留的抑制劑成分。樣品溶解在ddH2O中，如果不是，請(qǐng)注明溶解液成分。</p><p>2. 確定STR位點(diǎn)，設(shè)計(jì)實(shí)驗(yàn)方案，包括熒光標(biāo)記的選擇，引物的設(shè)計(jì)、合成和標(biāo)記，熒光分子量?jī)?nèi)標(biāo)的選用等。</p><p>3. 按照所設(shè)計(jì)的實(shí)驗(yàn)方案完成熒光PCR，利用瓊脂糖電泳檢測(cè)結(jié)果；若PCR產(chǎn)物特異性不夠，將進(jìn)行優(yōu)化。</p><p>4. 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