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<strong>DNA抽提和純化</strong>主要是CTAB方法，其他的方法還有物理方式如玻璃珠法、超聲波法、研磨法、凍融法?；瘜W方式如異硫氰酸胍法、堿裂解法。生物方式：酶法。根據(jù)核酸分離純化方式的不同有；硅質材料、陰離子交換樹脂等。<br /><br type="_moz" /></span></span></span></span><div><span style="color: rgb(0, 0, 0);"><span style="font-size: small;"><span style="font-family: Arial;">　　DNA抽提和純化總的原則：</span></span></span></div><div><span style="color: rgb(0, 0, 0);"><span style="font-size: small;"><span style="font-family: Arial;">　　1、保證核酸一級結構的完整性；</span></span></span></div><div><span style="color: rgb(0, 0, 0);"><span style="font-size: small;"><span style="font-family: Arial;">　　2、核酸樣品中不應存在對酶有抑制作用的有機溶劑和過高濃度的金屬離子；</span></span></span></div><div><span style="color: rgb(0, 0, 0);"><span style="font-size: small;"><span style="font-family: Arial;">　　3、其他生物大分子如蛋白質、多糖和脂類分子的污染應降低到zui低程度。<br /><br type="_moz" /></span></span></span></div><div><span style="color: rgb(0, 0, 0);"><span style="font-size: small;"><span style="font-family: Arial;">　　<strong>方法</strong>介紹：</span></span></span></div><div><span style="color: rgb(0, 0, 0);"><span style="font-size: small;"><span style="font-family: Arial;">　　一、酚抽提法：先用蛋酶K、SDS破碎細胞，消化蛋白，然后用酚和酚-氯DNA大小為100-150kb</span></span></span></div><div><span style="color: rgb(0, 0, 0);"><span style="font-size: small;"><span style="font-family: Arial;">　　二、甲酰胺解聚法：破碎細胞同上，然后用高濃度甲酰胺解聚蛋白質與DNA的結合，再透析獲得DNA可得DNA200kb左右。</span></span></span></div><div><span style="color: rgb(0, 0, 0);"><span style="font-size: small;"><span style="font-family: Arial;">　　三、玻璃棒纏繞法：用鹽酸胍裂解細胞，將裂解物鋪于乙醇上，然后用帶鉤或U型玻璃棒在界面輕攪，DAN沉淀液繞于玻棒。生成DNA約80kb。</span></span></span></div><div><span style="color: rgb(0, 0, 0);"><span style="font-size: small;"><span style="font-family: Arial;">　　四、異丙醇沉淀法：基本同1法，僅用二倍容積異丙醇替代乙醇，可去除小分子RNA（在異丙醇中可溶狀態(tài)）</span></span></span></div><div><span style="color: rgb(0, 0, 0);"><span style="font-size: small;"><span style="font-family: Arial;">　　五、表面活性劑快速制備法：用Triton X-100A或NP40表面活性劑破碎細胞，然后用蛋白酶K或酚去除蛋白，乙醇沉淀或透析。</span></span></span></div><div><span style="color: rgb(0, 0, 0);"><span style="font-size: small;"><span style="font-family: Arial;">　　六、加熱法快速制備：加熱96℃-100℃，五分鐘，然后離心后取上清，可用于PCR反應。</span></span></span></div><div><span style="color: rgb(0, 0, 0);"><span style="font-size: small;"><span style="font-family: Arial;">　　七、堿變性快速制備：先用NaOH作用20分鐘，再加HCI中和，離心后取上清，含少量DNA。</span></span></span></div></div> <div class="x4vfcpe" id="nr_textbox"><link rel="stylesheet" type="text/css" href="/css/MessageBoard_style.css"> <script language="javascript" src="/skins/Scripts/order.js?v=20210318" type="text/javascript"></script> <a name="order" id="order"></a> <div id="x4vfcpe" class="ly_msg" id="ly_msg"> <form method="post" name="form2" id="form2"> <h3>留言框 </h3> <ul> <li> <h4 class="xh">產(chǎn)品：</h4> <div id="x4vfcpe" class="msg_ipt1"><input class="textborder" size="30" name="Product" id="Product" value="DNA抽提和純化" placeholder="請輸入產(chǎn)品名稱" /></div> </li> <li> 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