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src="/Skins/270236/images/dot.png" /><span>更新時(shí)間：</span>2024-05-13</li> <li><img src="/Skins/270236/images/dot.png" /><span>訪問量：</span>3513</li> </ul> <div id="x4vfcpe" class="pro_detail_btn"> <a href="#order" class="prodtl_btn1">產(chǎn)品咨詢</a><a href="/contact.html" target="_blank" class="prodtl_btn2">聯(lián)系我們</a> </div> </div> <div id="x4vfcpe" class="clear"></div> </div>  <div class="x4vfcpe" id="ny_pro_box02"> <div class="x4vfcpe" id="con"> <ul id="tags"> <li id="x4vfcpe" class=selectTag><A onmouseover="selectTag('tagContent0',this)" href="javascript:void(0)"onFocus="this.blur()">產(chǎn)品介紹：</A> </li> </ul> <div class="x4vfcpe" id=tagContent> <div id="x4vfcpe" class="tagContent selectTag" id=tagContent0><h2 style="text-align:center"><span style="color:#013ca0"><span style="font-family:微軟雅黑,sans-serif"><span style="font-size:22.5pt">CRISPR-Cas9</span></span></span><span style="color:#013ca0"><span style="font-family:微軟雅黑,sans-serif"><span style="font-size:22.5pt">*基因敲除小鼠</span></span></span></h2><p><span style="color:#555555"><span style="font-family:微軟雅黑,sans-serif">CRISPR-Cas9</span></span><span style="color:#555555"><span style="font-family:微軟雅黑,sans-serif">核酸酶技術(shù)，通過體外轉(zhuǎn)錄，獲得編碼Cas9酶與SgRNA，然后通過受精卵顯微注射，獲得基因敲除小鼠模型, 為快速制備基因敲除小鼠開辟了新的技術(shù)途徑。</span></span></p><p><span style="color:#555555"><span style="font-family:微軟雅黑,sans-serif">基屹生物可以根據(jù)客戶的需求設(shè)計(jì)高效特異的3-5條Guide RNA，并通過細(xì)胞實(shí)驗(yàn)篩選的序列，保證成功率。整個(gè)實(shí)驗(yàn)流程僅需3-5個(gè)月。</span></span></p><p> <strong><span style="color:#3333FF"><span style="font-family:微軟雅黑,sans-serif">利用CRISPR-Cas9技術(shù)制備基因打靶小鼠的原理圖：</span></span></strong></p><p><span style="color:#555555"><span style="font-family:微軟雅黑,sans-serif">在沒有外加供體模板DNA的情況下，細(xì)胞在CRISPR-Cas9剪切目的基因后，通過NHEJ途徑產(chǎn)生移碼突變，從而獲得基因敲除小鼠；在外加供體模板DNA后，細(xì)胞在CRISPR-Cas9剪切目的基因后，通過HDR途徑將外源DNA序列引入到基因組中，從而獲得基因敲入小鼠或條件型基因敲除小鼠。</span></span></p><p style="text-align:center"><span style="color:#555555"><span 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