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class="pro_detail_btn"> <a href="#order" class="prodtl_btn1">產(chǎn)品咨詢</a><a href="/contact.html" target="_blank" class="prodtl_btn2">聯(lián)系我們</a> </div> </div> <div id="x4vfcpe" class="clear"></div> </div>  <div class="x4vfcpe" id="ny_pro_box02"> <div class="x4vfcpe" id="con"> <ul id="tags"> <li id="x4vfcpe" class=selectTag><A onmouseover="selectTag('tagContent0',this)" href="javascript:void(0)"onFocus="this.blur()">產(chǎn)品介紹：</A> </li> </ul> <div class="x4vfcpe" id=tagContent> <div id="x4vfcpe" class="tagContent selectTag" id=tagContent0><p><strong>siRNA干擾檢測(cè)服務(wù)廠家</strong></p><p>原理：RNA干擾 (RNA interference, RNAi)技術(shù)，是將與內(nèi)源mRNA 編碼區(qū)同源的小片段(19 -23bp)外源雙鏈 RNA(siRNA)導(dǎo)入細(xì)胞中，引發(fā)細(xì)胞中相應(yīng)mRNA 高效特異性降解，從而導(dǎo)致特定基因表達(dá)沉默(knock down)的一種實(shí)驗(yàn)技術(shù)，其中通過構(gòu)建表達(dá)載體表達(dá)siRNA是多種siRNA制備方法中有可能進(jìn)行長(zhǎng)期基因沉默研究的方法，siRNA 載體可以在細(xì)胞內(nèi)直接轉(zhuǎn)錄出shrna ，其干擾效果等同于siRNA ，而且能夠解決 siRNA干擾時(shí)間短的缺點(diǎn)。您只需提供需干擾基因的詳細(xì)信息，包括基因的 mRNA 序列，Genbank Accession No. 和所屬物種，本公司負(fù)責(zé)設(shè)計(jì)3條針對(duì)目的mRNA的siRNA 靶序列，在短時(shí)間內(nèi)構(gòu)建三個(gè)可用于目的mRNA干擾實(shí)驗(yàn)的siRNA 載體并為您進(jìn)行后續(xù)的干擾實(shí)驗(yàn)研究。</p><p><strong>siRNA干擾檢測(cè)服務(wù)廠家</strong>實(shí)驗(yàn)流程</p><p>◇ 選擇siRNA表達(dá)載體通過人工合成基因的方法將該序列克隆至Clontech公司的RNAi-Ready pSIREN系列表達(dá)載體中，該系列表達(dá)載體帶有Pol III(U6)啟動(dòng)子，具有轉(zhuǎn)染效率高、操作簡(jiǎn)單、rna沉默效果易于檢測(cè)等特點(diǎn)，客戶可根據(jù)需要進(jìn)行選擇。</p><p><strong>siRNA干擾檢測(cè)服務(wù)</strong> (RNA interference, RNAi)技術(shù)，是將與內(nèi)源mRNA 編碼區(qū)同源的小片段(19 -23bp)外源雙鏈 RNA(siRNA)導(dǎo)入細(xì)胞中，引發(fā)細(xì)胞中相應(yīng)mRNA 高效特異性降解，從而導(dǎo)致特定基因表達(dá)沉默(knock down)的一種實(shí)驗(yàn)技術(shù)。</p><p> </p><p>◇ 根據(jù)客戶提交的目的mRNA序列，設(shè)計(jì)3條RNA干擾靶序列，靶序列一般為19-21nt 長(zhǎng)。</p><p>◇ 對(duì)于每條選定的siRNA 靶序列，設(shè)計(jì) siRNA 正義鏈和反義鏈，以 loop(9nt or 10nt) 相連，形成穩(wěn)定的發(fā)卡RNA，稱為 shRNA(short hairpin RNA)。</p><p>◇ 合成模板合成每條編碼 shRNA的DNA 模板，并連接到 siRNA 載體相應(yīng)的多克隆位點(diǎn)之間。連接產(chǎn)物轉(zhuǎn)化DH5α大腸桿菌。</p><p>◇ 抽提陽性克隆質(zhì)粒公司試劑盒抽提陽性克隆載體并定量。</p><p>◇ 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