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熒光定量PCR檢測技術(shù)服務(wù)-Taqman探針法 </span></div> <div id="priymv99ks" class="about"> <div id="priymv99ks" class="PageView"> <div class="priymv99ks" id="nr_main_box">  <div id="priymv99ks" class="pro_detail_main"> <div id="priymv99ks" class="prodetail_img"> <div class="priymv99ks" id="preview"> <div id="priymv99ks" class="jqzoom" id="spec-n1"> <div class="priymv99ks" id="ceshi" style=""> <img src="http://img51.chem17.com/gxhpic_33572a5ad1/c27d282b725efa31c131bcf5ef0386d020122eaa4374f7667300cbfa08284d256b97cd62defe24e4.png" jqimg="http://img51.chem17.com/gxhpic_33572a5ad1/c27d282b725efa31c131bcf5ef0386d020122eaa4374f7667300cbfa08284d256b97cd62defe24e4.png" /> </div> </div> <div class="priymv99ks" id="spec-n5">  <div class="priymv99ks" id="spec-list"> <ul class="list-h" > <li><p><img onclick="jQuery('.videoItem').hide();mybaidu.window.SetPause();" src="http://img51.chem17.com/gxhpic_33572a5ad1/c27d282b725efa31c131bcf5ef0386d020122eaa4374f7667300cbfa08284d256b97cd62defe24e4_800_800_5.png" 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class="prodtl_btn1">產(chǎn)品咨詢</a><a href="/contact.html" target="_blank" class="prodtl_btn2">聯(lián)系我們</a> </div> </div> <div id="priymv99ks" class="clear"></div> </div>  <div class="priymv99ks" id="ny_pro_box02"> <div class="priymv99ks" id="con"> <ul id="tags"> <li id="priymv99ks" class=selectTag><A onmouseover="selectTag('tagContent0',this)" href="javascript:void(0)"onFocus="this.blur()">產(chǎn)品介紹：</A> </li> </ul> <div class="priymv99ks" id=tagContent> <div id="priymv99ks" class="tagContent selectTag" id=tagContent0><p><strong>實(shí)時(shí)熒光定量PCR技術(shù)（Real Time Quantitative PCR）</strong></p><p>在PCR反應(yīng)體系中加入熒光基團(tuán)，利用熒光信號(hào)積累實(shí)時(shí)監(jiān)測整個(gè)PCR進(jìn)程，zui后通過標(biāo)準(zhǔn)曲線對(duì)未知模板進(jìn)行定量分析的方法。</p><p>相對(duì)定量應(yīng)用（Relative Quantification，RQ） </p><p> 特異性熒光標(biāo)記：</p><p> TaqMan法</p><p> TaqMan探針的特性：</p><p>（1）5′端標(biāo)記有報(bào)告基團(tuán)（Reporter, R），如FAM、VIC等</p><p>（2）3′端標(biāo)記有熒光淬滅基團(tuán) （Quencher, Q）</p><p>（3）探針完整，R所發(fā)射的熒光能量被Q基團(tuán)吸收，無熒光， R與Q分開，發(fā)熒光</p><p>（4）Taq酶有 5′→3′外切核酸酶活性，可水解探針</p><p><strong>相對(duì)定量通過內(nèi)標(biāo)定量：</strong><br />內(nèi)標(biāo)（Endogenous Control）通常是β-actin、GAPDH基因等看家基因，在細(xì)胞中的表達(dá)量或在基因組中的拷貝數(shù)恒定，受環(huán)境因素影響小，內(nèi)標(biāo)定量結(jié)果代表了樣本中所含細(xì)胞或基因組數(shù)量。</p><p><strong>相對(duì)定量分析方法：</strong></p><p> 2-ΔΔCt<br />前提：目標(biāo)序列和內(nèi)參序列的擴(kuò)增效率接近100％且偏差在5％以內(nèi)<br /> 1、用內(nèi)參基因的CT值歸一目標(biāo)基因的CT值：<br /> ΔCT（test）= CT（target,test）-CT（ref,test） <br /> ΔCT（calibrator）= CT（target,calibrator）-CT（ref, calibrator） <br /> 2、用校準(zhǔn)樣本的ΔCT值歸一試驗(yàn)樣本的ΔCT值：<br /> ΔΔCT= ΔCT（test） - ΔCT（calibrator）<br /> 3、計(jì)算表達(dá)水平比率：<br /> 2 –ΔΔCT=表達(dá)量的比值</p><p><strong>服務(wù)流程：</strong></p><table border="0" cellpadding="0" cellspacing="0"><tbody><tr><td><p>步驟</p></td><td><p>客戶提供</p></td><td><p>服務(wù) 內(nèi) 容</p></td><td><p>基屹提供</p></td></tr><tr><td><p>1</p></td><td><p>新鮮的且盡量多的材料；已知的全長基因序列；盡可能詳細(xì)的背景資料：DNA/ RNA來源、豐度等。</p></td><td><p>DNA/RNA提取，根據(jù)基因序列設(shè)計(jì)特異性的引物和熒光探針。</p></td><td> </td></tr><tr><td><p>2</p></td><td><p>純化好的DNA/RNA（大于5 ug/樣品）；已知的全長基因序列；盡可能詳細(xì)的背景資料：DNA/ RNA來源、豐度等。</p></td><td><p>根據(jù)基因序列設(shè)計(jì)特異性的引物和熒光探針。</p></td><td> </td></tr><tr><td><p>3</p></td><td> </td><td><p>以DNA為模板，對(duì)目的基因進(jìn)行熒光定量PCR檢測分析</p></td><td><p>提供完整的定量分析結(jié)果、實(shí)驗(yàn)熒光定量擴(kuò)增曲線及詳細(xì)的實(shí)驗(yàn)步驟。</p></td></tr></tbody></table><p>注意：客戶可根據(jù)所能提供的起始材料不同，選擇從步驟1或2啟動(dòng)項(xiàng)目。</p><p> </p><p> </p></div> <div class="priymv99ks" id="nr_textbox"><link rel="stylesheet" type="text/css" href="/css/MessageBoard_style.css"> <script language="javascript" src="/skins/Scripts/order.js?v=20210318" type="text/javascript"></script> <a name="order" id="order"></a> <div id="priymv99ks" class="ly_msg" id="ly_msg"> <form method="post" name="form2" id="form2"> <h3>留言框 </h3> <ul> <li> <h4 class="xh">產(chǎn)品：</h4> <div id="priymv99ks" class="msg_ipt1"><input class="textborder" size="30" name="Product" id="Product" value="熒光定量PCR檢測技術(shù)服務(wù)-Taqman探針法" 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