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/><span>更新時(shí)間：</span>2024-05-13</li> <li><img src="/Skins/270236/images/dot.png" /><span>訪問(wèn) 量：</span>3754</li> </ul> <div id="x4vfcpe" class="pro_detail_btn"> <a href="#order" class="prodtl_btn1">產(chǎn)品咨詢</a><a href="/contact.html" target="_blank" class="prodtl_btn2">聯(lián)系我們</a> </div> </div> <div id="x4vfcpe" class="clear"></div> </div>  <div class="x4vfcpe" id="ny_pro_box02"> <div class="x4vfcpe" id="con"> <ul id="tags"> <li id="x4vfcpe" class=selectTag><A onmouseover="selectTag('tagContent0',this)" href="javascript:void(0)"onFocus="this.blur()">產(chǎn)品介紹：</A> </li> </ul> <div class="x4vfcpe" id=tagContent> <div id="x4vfcpe" class="tagContent selectTag" id=tagContent0><p><strong>定量（Absolute Quantification，AQ）</strong></p><p>分析用于確定未知樣本中某個(gè)核酸序列的量值，即通常所說(shuō)的拷貝數(shù)，應(yīng)用與病原體檢測(cè)，轉(zhuǎn)基因食品檢測(cè)，及基因表達(dá)研究。</p><p><strong>SYBR Green特性：</strong></p><p>（1）只有和雙鏈DNA結(jié)合后才發(fā)熒光。</p><p>（2）變性時(shí)，DNA雙鏈分開(kāi)，無(wú)熒光。</p><p>（3）在延伸結(jié)束階段采集熒光信號(hào)。</p><p>（4）SYBR Green也能和非特異性的雙鏈DNA結(jié)合發(fā)光，所以必須在反應(yīng)結(jié)束時(shí)做熔解曲線分析。 </p><p><strong>TaqMan探針的特性：</strong></p><p>（1）5′端標(biāo)記有報(bào)告基團(tuán)（Reporter, R），如FAM、VIC等 </p><p>（2）3′端標(biāo)記有熒光淬滅基團(tuán) （Quencher, Q）</p><p>（3）探針完整，R所發(fā)射的熒光能量被Q基團(tuán)吸收，無(wú)熒光， R與Q分開(kāi)，發(fā)熒光（4）Taq酶有 5′→3′外切核酸酶活性，可水解探針。</p><p><strong>定量的標(biāo)準(zhǔn)樣品</strong>：</p><p>（1）含有和待測(cè)樣品相同擴(kuò)增片段的克隆質(zhì)粒</p><p>（2）含有和待測(cè)樣品相同擴(kuò)增片段的cDNA</p><p>（3）PCR的產(chǎn)物，可分別做系列稀釋。</p><p><strong>服務(wù)流程：</strong></p><table><tbody><tr><td><p>步驟</p></td><td><p>客戶提供</p></td><td><p>服務(wù) 內(nèi) 容</p></td><td><p>基屹提供</p></td></tr><tr><td><p>1</p></td><td><p>新鮮的且盡量多的材料；已知的全長(zhǎng)基因序列；盡可能詳細(xì)的背景資料：DNA/ RNA來(lái)源、豐度等。</p></td><td><p>DNA/RNA提取，根據(jù)基因序列設(shè)計(jì)特異性的引物和熒光探針。</p></td><td><p> </p></td></tr><tr><td><p>2</p></td><td><p>純化好的DNA/RNA（大于5 ug/樣品）；已知的全長(zhǎng)基因序列；盡可能詳細(xì)的背景資料：DNA/ RNA來(lái)源、豐度等。</p></td><td><p>根據(jù)基因序列設(shè)計(jì)特異性的引物和熒光探針。</p></td><td><p> </p></td></tr><tr><td><p>3</p></td><td><p> </p></td><td><p>以DNA為模板，對(duì)目的基因進(jìn)行熒光定量PCR檢測(cè)分析</p></td><td><p>提供完整的定量分析結(jié)果、實(shí)驗(yàn)熒光定量擴(kuò)增曲線及詳細(xì)的實(shí)驗(yàn)步驟。</p></td></tr></tbody></table><p>注意：客戶可根據(jù)所能提供的起始材料不同，選擇從步驟1或2啟動(dòng)項(xiàng)目。</p><p> </p><p> </p><p> </p></div> <div class="x4vfcpe" id="nr_textbox"><link rel="stylesheet" type="text/css" href="/css/MessageBoard_style.css"> <script language="javascript" src="/skins/Scripts/order.js?v=20210318" type="text/javascript"></script> <a name="order" id="order"></a> <div id="x4vfcpe" class="ly_msg" id="ly_msg"> <form method="post" name="form2" id="form2"> <h3>留言框 </h3> <ul> <li> <h4 class="xh">產(chǎn)品：</h4> <div id="x4vfcpe" class="msg_ipt1"><input class="textborder" size="30" name="Product" id="Product" value="熒光定量PCR技術(shù)服務(wù)-定量" placeholder="請(qǐng)輸入產(chǎn)品名稱" /></div> </li> <li> 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