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type=text/javascript src="/Skins/270236/js/lib.js"></script> <script type=text/javascript src="/Skins/270236/js/zzsc.js"></script> </div> <div id="x4vfcpe" class="pro_detail_text"> <h1>Lentiviral 慢病毒制備</h1> <div id="x4vfcpe" class="jyms"><p><span>簡要描述：</span>慢病毒是逆轉(zhuǎn)錄病毒的一種，具有逆轉(zhuǎn)錄病毒的基本結(jié)構(gòu)和特性：整合入宿主細(xì)胞基因組，長期表達(dá)，可用于轉(zhuǎn)基因動(dòng)物制備；但也有不同于逆轉(zhuǎn)錄病毒的組份和特性：比逆轉(zhuǎn)錄病毒具有更高的滴度，不僅可以感染分裂期細(xì)胞，更重要的，還能感染非分裂期的細(xì)胞。利用慢病毒載體，可以研究啟動(dòng)子的調(diào)控、過表達(dá)特定基因或沉默特定基因。</p></div> <ul> <li><img src="/Skins/270236/images/dot.png" /><span>產(chǎn)品型號(hào)：</span></li> <li><img src="/Skins/270236/images/dot.png" /><span>廠商性質(zhì)：</span>生產(chǎn)廠家</li> <li><img src="/Skins/270236/images/dot.png" /><span>更新時(shí)間：</span>2024-05-13</li> <li><img src="/Skins/270236/images/dot.png" /><span>訪問量：</span>1951</li> </ul> <div id="x4vfcpe" class="pro_detail_btn"> <a href="#order" class="prodtl_btn1">產(chǎn)品咨詢</a><a href="/contact.html" target="_blank" class="prodtl_btn2">聯(lián)系我們</a> </div> </div> <div id="x4vfcpe" class="clear"></div> </div>  <div class="x4vfcpe" id="ny_pro_box02"> <div class="x4vfcpe" id="con"> <ul id="tags"> <li id="x4vfcpe" class=selectTag><A onmouseover="selectTag('tagContent0',this)" href="javascript:void(0)"onFocus="this.blur()">產(chǎn)品介紹：</A> </li> </ul> <div class="x4vfcpe" id=tagContent> <div id="x4vfcpe" class="tagContent selectTag" id=tagContent0><p>慢病毒包裝服務(wù)</p><p>慢病毒簡介</p><p> 慢病毒是逆轉(zhuǎn)錄病毒的一種，具有逆轉(zhuǎn)錄病毒的基本結(jié)構(gòu)和特性：整合入宿主細(xì)胞基因組，長期表達(dá)，可用于轉(zhuǎn)基因動(dòng)物制備；但也有不同于逆轉(zhuǎn)錄病毒的組份和特性：比逆轉(zhuǎn)錄病毒具有更高的滴度，不僅可以感染分裂期細(xì)胞，更重要的，還能感染非分裂期的細(xì)胞。利用慢病毒載體，可以研究啟動(dòng)子的調(diào)控、過表達(dá)特定基因或沉默特定基因。</p><p>慢病毒包裝技術(shù)特點(diǎn)</p><table><tbody><tr><td><p>病毒表達(dá)系統(tǒng)</p></td><td><p>腺病毒表達(dá)系統(tǒng)</p></td><td><p>慢病毒表達(dá)系統(tǒng)</p></td></tr><tr><td><p>病毒基因組</p></td><td><p>雙鏈DNA病毒</p></td><td><p>RNA逆轉(zhuǎn)錄病毒</p></td></tr><tr><td><p>自主復(fù)制</p></td><td><p>不能</p></td><td><p>不能</p></td></tr><tr><td><p>是否整合</p></td><td><p>病毒基因組游離于宿主基因組外，瞬時(shí)表達(dá)外源基因</p></td><td><p>病毒基因組整合于宿主基因組，長時(shí)間、穩(wěn)定表達(dá)外源基因</p></td></tr><tr><td><p>感染細(xì)胞類型</p></td><td><p>感染絕大多數(shù)細(xì)胞，包括分裂期、非分裂期細(xì)胞。</p></td><td><p>感染絕大多數(shù)細(xì)胞，包括分裂期、非分裂期細(xì)胞。</p></td></tr><tr><td><p>表達(dá)豐度</p></td><td><p>高水平表達(dá)</p></td><td><p>中水平表達(dá)</p></td></tr><tr><td><p>表達(dá)時(shí)間</p></td><td><p>快（1-2 天）</p></td><td><p>慢（2-4 天）</p></td></tr><tr><td><p>滴度</p></td><td><p>滴度可達(dá)10E12pfu/mL</p></td><td><p>滴度可達(dá)10E9－10E10TU/mL</p></td></tr><tr><td><p>克隆容量</p></td><td><p>可插入高達(dá)8kb的外源片段，滴度隨插入片段長度增加而降低</p></td><td><p>可插入不超過8kb的外源片段，滴度隨插入片段長度增加而降低</p></td></tr><tr><td><p>免疫原性</p></td><td><p>高免疫原性</p></td><td><p>低免疫原性</p></td></tr><tr><td><p>RNAi</p></td><td><p>病毒進(jìn)入細(xì)胞往往引起細(xì)胞內(nèi)干擾素反應(yīng)，一般情況下不適合做RNAi實(shí)驗(yàn)</p></td><td><p>適合，是RNAi實(shí)驗(yàn)的優(yōu)選工具</p></td></tr><tr><td><p>基因過表達(dá)</p></td><td><p>包裝容量較大，可以滿足較大基因的包裝，是過表達(dá)基因的優(yōu)選工具</p></td><td><p>包裝容量有限，過表達(dá)的基因過大時(shí)，病毒滴度受到影響</p></td></tr><tr><td><p>過表達(dá)microRNA</p></td><td><p>適合</p></td><td><p>適合，是過表達(dá)microRNA實(shí)驗(yàn)的優(yōu)選工具</p></td></tr><tr><td><p>下調(diào)microRNA</p></td><td><p>有報(bào)道但不成熟</p></td><td><p>有報(bào)道但不成熟</p></td></tr><tr><td><p>是否可以自行擴(kuò)增</p></td><td><p>可以</p></td><td><p>需要特殊包裝系統(tǒng)和包裝環(huán)境</p></td></tr></tbody></table><p>A. 慢病毒載體的構(gòu)建：</p><p>根據(jù)不同的研究需求構(gòu)建慢病毒載體，如用于RNAi的慢病毒載體、用于基因過表達(dá)的慢病毒載體、用于啟動(dòng)子活性研究的慢病毒載體等；</p><p>B. 慢病毒包裝過程：</p><p> 將重組病毒質(zhì)粒及其輔助包裝質(zhì)粒共轉(zhuǎn)染至包裝細(xì)胞中，收集富含慢病毒顆粒的細(xì)胞上清液，對(duì)其濃縮后得到高滴度的慢病毒濃縮液，在293T細(xì)胞中測定并標(biāo)定病毒滴度。</p><p>C. 慢病毒包裝結(jié)果：</p><p>滴定濃度：1×107～1×108TU/ml</p><p>zui終體積：1 ml純化后的病毒液（儲(chǔ)存于PBS）</p><p>儲(chǔ)存：批量儲(chǔ)存的*條件為-80°C。</p><p>慢病毒包裝用途：</p><p>慢病毒載體包裝之后成為假病毒顆粒后，可用于:</p><p>· 感染原代細(xì)胞；</p><p>· 制備穩(wěn)定表達(dá)/沉默特定基因的單克隆細(xì)胞株；</p><p>· in vivo的基因操作；</p><p>· 轉(zhuǎn)基因動(dòng)物，特別是轉(zhuǎn)基因大鼠的制備。</p></div> <div class="x4vfcpe" id="nr_textbox"><link rel="stylesheet" type="text/css" href="/css/MessageBoard_style.css"> <script language="javascript" src="/skins/Scripts/order.js?v=20210318" type="text/javascript"></script> <a name="order" id="order"></a> <div id="x4vfcpe" class="ly_msg" id="ly_msg"> <form method="post" name="form2" id="form2"> <h3>留言框 </h3> <ul> <li> <h4 class="xh">產(chǎn)品：</h4> <div id="x4vfcpe" class="msg_ipt1"><input 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