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class="jyms"><p><span>簡要描述：</span>四甲基偶氮唑鹽比色法（MTT方法），是通過快速簡便的顏色反應(yīng)來檢測細(xì)胞存活數(shù)量。其原理是MTT可作為哺乳類動(dòng)物細(xì)胞線粒體中琥珀酸脫氫酶的底物。當(dāng)有活細(xì)胞存在時(shí)，線粒體內(nèi)琥珀酸脫氫酶可將淡黃色的MTT還原成藍(lán)紫色的針狀甲月替(Formazan)結(jié)晶并沉積在細(xì)胞中，結(jié)晶物能被二甲基亞砜 (DMSO)溶解,用酶聯(lián)免疫檢測儀在570nm波長處測定其吸光度OD值，OD值的高低可間接反映活細(xì)胞的數(shù)量及其活性。</p></div> <ul> <li><img src="/Skins/270236/images/dot.png" /><span>產(chǎn)品型號(hào)：</span></li> <li><img src="/Skins/270236/images/dot.png" /><span>廠商性質(zhì)：</span>生產(chǎn)廠家</li> <li><img src="/Skins/270236/images/dot.png" /><span>更新時(shí)間：</span>2024-05-13</li> <li><img src="/Skins/270236/images/dot.png" /><span>訪問量：</span>2685</li> </ul> <div id="x4vfcpe" class="pro_detail_btn"> <a href="#order" class="prodtl_btn1">產(chǎn)品咨詢</a><a href="/contact.html" target="_blank" class="prodtl_btn2">聯(lián)系我們</a> </div> </div> <div id="x4vfcpe" class="clear"></div> </div>  <div class="x4vfcpe" id="ny_pro_box02"> <div class="x4vfcpe" id="con"> <ul id="tags"> <li id="x4vfcpe" class=selectTag><A onmouseover="selectTag('tagContent0',this)" href="javascript:void(0)"onFocus="this.blur()">產(chǎn)品介紹：</A> </li> </ul> <div class="x4vfcpe" id=tagContent> <div id="x4vfcpe" class="tagContent selectTag" id=tagContent0><p><strong>MTT細(xì)胞活性檢測服務(wù)</strong></p><p>四甲基偶氮唑鹽比色法（MTT方法），是通過快速簡便的顏色反應(yīng)來檢測細(xì)胞存活數(shù)量。其原理是MTT可作為哺乳類動(dòng)物細(xì)胞線粒體中琥珀酸脫氫酶的底物。當(dāng)有活細(xì)胞存在時(shí)，線粒體內(nèi)琥珀酸脫氫酶可將淡黃色的MTT還原成藍(lán)紫色的針狀甲月替（Formazan）結(jié)晶并沉積在細(xì)胞中，結(jié)晶物能被二甲基亞砜（DMSO）溶解,用酶聯(lián)免疫檢測儀在570nm波長處測定其吸光度OD值，OD值的高低可間接反映活細(xì)胞的數(shù)量及其活性。</p><p><strong>MTT法常用的研究：</strong></p><p>1、檢測細(xì)胞活性：常作為細(xì)胞毒性試驗(yàn)的一種方法。</p><p>2、體外藥物敏感實(shí)驗(yàn)：該方法簡便、經(jīng)濟(jì)、快速，重復(fù)性好，所需的細(xì)胞數(shù)較少，沒有放射性，目前廣泛的用于臨床前的抗癌藥物的篩選研究。</p><p>3、一些細(xì)胞因子活性的研究</p><p><strong>實(shí)驗(yàn)步驟:</strong></p><p>1）將對(duì)數(shù)生長期細(xì)胞用胰酶消化后配制成濃度為1-10×104個(gè)/ml的細(xì)胞懸液，按1000-10000個(gè)細(xì)胞/孔接種于96孔板，每孔加100μl。</p><p>2）將平板置37℃、 5%CO2濕度培養(yǎng)箱中24小時(shí)后，加入含有不同濃度受試樣本的培養(yǎng)液100μl，每個(gè)樣本不同濃度設(shè)平行的三個(gè)孔，對(duì)照組加不含樣本的培養(yǎng)液100μl，再放入培養(yǎng)箱中孵育24-72 小時(shí)，</p><p>3）用快速翻板法倒掉培養(yǎng)液，每孔加入用無血清1640按5mg/ml新鮮配制的MTT溶液100μl，溫育4小時(shí)，使MTT還原為甲月替，再次倒掉上清液，每孔加DMSO（二甲基亞楓）200μl，用平板搖床搖勻后，使用酶標(biāo)儀測定光密度值（OD）（參考波長450nm，檢測波長570nm），以溶劑對(duì)照處理細(xì)胞為對(duì)照組，按下述公式計(jì)算化合物對(duì)細(xì)胞的抑制率，并按中效方程計(jì)算半數(shù)抑制濃度IC50：</p><p><strong>客戶方需提供:</strong></p><p>樣本描述,相關(guān)的實(shí)驗(yàn)設(shè)計(jì)內(nèi)容如作用時(shí)間和樣本濃度等。</p><p><strong>公司方提供：</strong></p><p>實(shí)驗(yàn)結(jié)果IC50值。</p><p><strong>實(shí)驗(yàn)周期與費(fèi)用：</strong></p><p>實(shí)驗(yàn)費(fèi)用以樣本數(shù)和細(xì)胞株的種類數(shù)目為單位計(jì)算，實(shí)驗(yàn)周期為18個(gè)工作日，公司將在材料和預(yù)付的實(shí)驗(yàn)經(jīng)費(fèi)全部到位后25個(gè)工作日之內(nèi)提交實(shí)驗(yàn)結(jié)果。客戶需在收到實(shí)驗(yàn)結(jié)果后一周內(nèi)付清所有剩余款項(xiàng)。</p></div> <div class="x4vfcpe" id="nr_textbox"><link rel="stylesheet" type="text/css" href="/css/MessageBoard_style.css"> <script language="javascript" 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