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target="_blank" class="prodtl_btn2">聯系我們</a> </div> </div> <div id="x4vfcpe" class="clear"></div> </div>  <div class="x4vfcpe" id="ny_pro_box02"> <div class="x4vfcpe" id="con"> <ul id="tags"> <li id="x4vfcpe" class=selectTag><A onmouseover="selectTag('tagContent0',this)" href="javascript:void(0)"onFocus="this.blur()">產品介紹：</A> </li> </ul> <div class="x4vfcpe" id=tagContent> <div id="x4vfcpe" class="tagContent selectTag" id=tagContent0><p><strong>DNA甲基化修飾服務</strong>概述：</p><div>DNA甲基化是zui早發(fā)現的修飾途徑之一，大量研究表明，DNA甲基化能引起染色質結構、DNA構象、DNA穩(wěn)定性及DNA與蛋白質相互作用方式的改變，從而控制基因表達。</div><div>基因組DNA由A、T、C、G四種堿基組成，DNA鏈中含有很多CpG結構, 雙鏈DNA中CpG的兩個胞嘧啶（C, cytosine）的5 位碳原子通常被甲基化, 且兩個甲基集團在DNA 雙鏈大溝中呈特定三維結構?；蚪M中60%～ 90% 的CpG 都被甲基化，未甲基化的CpG 成簇地組成CpG 島, 位于結構基因啟動子的核心序列和轉錄起始點。</div><div>有實驗證明超甲基化阻遏轉錄的進行。DNA 甲基化可引起基因組中相應區(qū)域染色質結構變化，使DNA 失去核酶?限制性內切酶的切割位點, 以及DNA 酶的敏感位點, 使染色質高度螺旋化, 凝縮成團, 失去轉錄活性。5 位C 甲基化的胞嘧啶脫氨基生成胸腺嘧啶, 由此可能導致基因置換突變, 發(fā)生堿基錯配: T/G, 如果在細胞分裂過程中不被糾正,就會誘發(fā)遺傳病或癌癥, 而且, 生物體甲基化的方式是穩(wěn)定的、可遺傳的。</div><div>DNA甲基轉移酶催化CpG的甲基化反應，DNA 甲基轉移酶有兩種: 1) DNMT1, 持續(xù)性DNA 甲基轉移酶—— 作用于僅有一條鏈甲基化的DNA 雙鏈, 使其*甲基化, 可參與DNA 復制雙鏈中的新合成鏈的甲基化,DNMT1 可能直接與HDAC (組蛋白去乙?；D移酶) 聯合作用阻斷轉錄; 2)DNMT3a、DNMT3b從頭甲基轉移酶, 它們可甲基化CpG, 使其半甲基化, 繼而全甲基化。從頭甲基轉移酶可能參與細胞生長分化調控, 其中DNMT3b在腫瘤基因甲基化中起重要作用。<br/><br type="_moz"/></div><div><strong style="text-wrap: wrap;">DNA甲基化修飾服務</strong>去甲基化有兩種方式：</div><div>1) 被動途徑: 由于核因子N F 粘附甲基化的DNA , 使粘附點附近的DNA不能被*甲基化, 從而阻斷DNM T1 的作用;</div><div>2) 主動途徑: 是由去甲基酶的作用, 將甲基集團移去的過程。在DNA 甲基化阻遏基因表達的過程中, 甲基化CPG 粘附蛋白起著重要作用。</div></div> <div class="x4vfcpe" id="nr_textbox"><link rel="stylesheet" type="text/css" href="/css/MessageBoard_style.css"> <script language="javascript" src="/skins/Scripts/order.js?v=20210318" type="text/javascript"></script> <a name="order" id="order"></a> <div id="x4vfcpe" class="ly_msg" id="ly_msg"> <form method="post" name="form2" id="form2"> <h3>留言框 </h3> <ul> <li> <h4 class="xh">產品：</h4> <div id="x4vfcpe" class="msg_ipt1"><input class="textborder" 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